If ATP and NADPH are already used up at night, the plant will not be able to run the Calvin Cycle (the light-independent reactions) until they are produced again during daylight.ExplanationATP and NADPH are made in the light-dependent reactions of photosynthesis, which need sunlight.At night, without light, the chloroplasts can’t produce more ATP and NADPH.The Calvin Cycle depends on these molecules to convert carbon dioxide into glucose. If they’re gone, sugar production stops temporarily.The plant will then rely on stored sugars and starch from previous photosynthesis for energy until light returns.In short — at night, once ATP and NADPH are used up, photosynthetic sugar-making pauses, and the plant switches to using stored food for survival.