The correct answer is D. Milk production will be slightly increased.When a buffalo (or any livestock) is in estrus (heat), it shows several physical and behavioral signs indicating that it is ready to mate. These include,Licking and smelling other animals – This is a social and sexual behavior.Trying to mount other animals – This is a common sign of heat.Standing still when mounted – This is called the standing heat and is one of the clearest signs of estrus.However, increased milk production is not a sign of estrus. In fact, during estrus, some animals may even show a slight decrease in milk production due to hormonal changes and restlessness.