Limitation and Challenges of Gene TherapyThe main limitation of gene therapy is delivery—getting the correct gene safely and efficiently into the right cells. Immune reactions to the vectors (like viruses) used to deliver genes.Difficulty in targeting only the affected cells without harming others.Potential for unintended effects, such as insertional mutations that can cause cancer.High cost and complexity of treatment.So, while gene therapy is promising, these obstacles still limit its widespread and safe use.